FIGURE

Fig. 3

ID

ZDB-FIG-101123-58

Publication

Cruz et al., 2010 - Induction and patterning of trunk and tail neural ectoderm by the homeobox gene eve1 in zebrafish embryos

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Fig. 3

Eve1 induces hoxb1b expression via an RA signal. (A–J and M–S) Dorsal and (K and L) lateral views (where discernible, dorsal to the right) of zebrafish embryos fixed for in situ staining at 80% epiboly (A–L) and 60% epiboly (M–S) Injections are indicated at the bottom left of each panel, and genes analyzed are given at the top right. (A–D) Suppression of otx2 by eve1 does not depend on RA because it resists overexpression of the RA-metabolizing enzyme Cyp26a1. (E–H) Eve1-mediated induction of hoxb1b does not occur when eve1 and cyp26a1 mRNAs are coinjected (G), and cyp26a1 injection alone suppresses hoxb1b expression (H; only one of two cells was injected in this embryo). (I and J) Eve1 induces aldh1a2 expression. (K and L) Anterior expression of cyp26a1 is suppressed by eve1 but remains unaffected at the margin. (M–O) Injection of high concentrations of aldMO and eve1 mRNA (Materials and Methods). Eve1 cannot rescue hoxb1b expression in aldMO-injected embryos (O). (P–S) Injection of low concentrations of eve1MO (2 ng/nL) and aldMO showed synergism in the suppression of hoxb1b.

Expression Data

Genes:	aldh1a2 cyp26a1 hoxb1b otx2b
Fish:	WT WT + MO1-eve1 WT + MO5-aldh1a2
Knockdown Reagents:	MO1-eve1 MO5-aldh1a2
Anatomical Terms:	anterior presumptive neural plate neuroectoderm presumptive neural plate presumptive paraxial mesoderm
Stage:	75%-epiboly

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA