FIGURE

Fig. S5

ID

ZDB-FIG-101123-45

Publication

Kim et al., 2010 - PDGF signaling is required for epicardial function and blood vessel formation in regenerating zebrafish hearts

Other Figures

All Figure Page

Back to All Figure Page

Fig. S5

PDGF signaling is required for DNA synthesis in 4-dpa epicardial cultures. (A) Dose-dependent effect of PDGFR inhibitor on DNA synthesis as percentage of BrdU-positive cells. Error bars indicate SEM. *P < 0.05. (B) Monolayer of epicardial cells migrated out from the apex of 4-dpa regenerating hearts after 3 d in culture. BrdU was added to the culture at the same time as DMSO (n = 12 hearts), PDGFR inhibitor (2 μM, n = 11 hearts), ROCK inhibitor (2 μM, n = 9 hearts), or PI3K inhibitor (inh; 2 μM, n = 13 hearts) for 1 d (i.e., from day 3 to day 4 in culture). BrdU staining is shown in red, and DAPI staining is shown in blue. (Scale bar = 50 μm.) (C) DNA synthesis quantified as the percentage of BrdU-positive cells. Error bars indicate SEM. *P < 0.01.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA