FIGURE

Fig. 1

ID

ZDB-FIG-101122-3

Publication

Arrenberg et al., 2009 - Optical control of zebrafish behavior with halorhodopsin

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Fig. 1

Expression of NpHR in zebrafish. (A) Expression pattern of Gal4^s1101t; UAS:Kaede transgenic animals. Dorsal view (i), horizontal optical slice through the hindbrain (ii) and transverse section through the eyes and midbrain (iii) of 5 dpf animals. (B) Expression of NpHR in Gal4^s1101t; UAS:(e)NpHR-XFP animals. (i and ii) NpHR-mCherry and eNpHR-eYFP, respectively. Intracellular blebs (arrows) are labeled in (i). (C) Surface targeting of NpHR. Co-expression of membrane-bound Dendra-kras and NpHR-mCherry in Gal4^s1013t; UAS:Dendra-kras; UAS:NpHR-mCherry animals (i) reveals suboptimal surface targeting. Co-expression of eNpHR-eYFP and membrane-bound mCherry-kras in Gal4^s1013t; UAS:eNpHR-eYFP; UAS:mCherry-kras animals, shows complete surface targeting of eNpHR-eYFP. [Scale bars, 100 μm in (A); 5 μm in (B and C).]

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA