FIGURE

Fig. 5

ID

ZDB-FIG-101118-25

Publication

Schuster et al., 2010 - Glial cell line-derived neurotrophic factor defines the path of developing and regenerating axons in the lateral line system of zebrafish

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Fig. 5

Regenerating axons can be guided by interneuromast cells. (A) After ablation of glial cells in the foxd3:gfp line, new glial cells extend posteriorly (arrowheads). (B) Their path resembles that of interneuromast cells (arrowheads) as visualized by alkaline phosphatase labeling. (C and D) After glial ablation between L1 and L2 in 3-dpf Huc:kaede, Et20:gfp fish, axons regrow along the myoseptum up to LII.1 (C) and follow the interneuromast cells (arrows) up to L2 (arrowhead in D). (E) Regenerating axons in a 54-hpf Huc:kaede, cldnb:gfp fish where the nerve had been cut at 30 hpf. White bars indicate the position of the horizontal myoseptum. (F) Trail of interneuromast cells in the same embryo; (G) merge. (H) Expression of gdnf in the primordium (dotted outline) and in the interneuromast cells (arrows) as revealed by in situ hybridization. (Scale bar, 100 mm.) A and H were assembled from two consecutive levels in Z-stacks.

Expression Data

Genes:	gdnfa GFP Kaede
Fish:	WT zf15Tg rw0130aTg; sqet20Et
Condition:	physical alteration: anatomical structure
Anatomical Terms:	glial cell interneuromast cell neuron posterior lateral line primordium
Stage Range:	Long-pec to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA