FIGURE

Fig. S2

ID

ZDB-FIG-101105-42

Publication

Jurynec et al., 2010 - SHIP2, a factor associated with diet-induced obesity and insulin sensitivity, attenuates FGF signaling in vivo

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Fig. S2

Ability of ship2a MOs to inhibit translation in vivo. (A-F) Expression of GFP in 50% epiboly embryos that were injected at the one-cell stage with 52 UTR GFP mRNA. Embryos were injected with mRNA only (A,B), with mRNA and ship2a MO1 (C,D), or with mRNA and ship2a MO2 (E,F). GFP was detected by immunofluorescence staining. (A,C,E) Fluorescence images (identical exposures times) showing GFP immunostaining and (B,D,F) corresponding fields of embryos under bright field illumination. (G) Immunoblot to detect GFP expression relative to control protein Paf-1 expression in extracts of 50% epiboly embryos that were: not injected (WT), injected at the one-cell stage with 52 UTR GFP mRNA only (-), or injected at the one-cell stage with 52 UTR GFP mRNA along with ship2a MO1 (+MO1) or ship2a MO2 (+MO2).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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