Complexin 3/4 concentrates in pineal photoreceptor terminals concomitant with RIBEYE b. (A) A high-magnification confocal projection of zpr 1/FRet 43-positive pineal double cone photoreceptors (red) double-stained for complexin 3/4 (green) at 1.2 dpf shows low levels of complexin in photoreceptor terminals (arrows). Ventral is toward the top and lateral is toward the right. (B) A section from a different embryo at 1.2 dpf double-stained with anti-zpr 1/FRet 43 (red) and anti-RIBEYE b (green) shows three small RIBEYE puncta in a pineal photoreceptor terminal (arrow). (C) By 1.5 dpf, complexin 3/4 (green) is highly expressed in neuropil at the lateral border of the pineal organ, in both zpr 1/FRet 43-positive (red) and zpr 1/FRet 43-negative photoreceptor axons and terminals. (D) Several RIBEYE b puncta (green) are present in pineal photoreceptor axons and terminals at 1.5 dpf. Scale bar = 5 μm.
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