FIGURE

Fig. 2

ID

ZDB-FIG-100903-42

Publication

Wang et al., 2010 - Moesin1 and Ve-cadherin are required in endothelial cells during in vivo tubulogenesis

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Fig. 2

α-Catenin-EGFP localizes to adherens junctions associated with the primary lumen in the ISVs. (A-C) Confocal images of the ISVs in a living Tg(flk1:α-catenin-egfp)/Tg(flk1:mCherry) zebrafish embryo at 30 hpf. Arrows in C point to long putative adherens junctions between cells. (D-F) Labeling of adherens junctions in the ISVs with anti-Ve-cadherin antibody in a Tg(flk1:α-catenin-egfp)/Tg(flk1:nlsmCherry) double-transgenic embryo. (G) Time-lapse confocal images of an ISV in a Tg(flk1:α-catenin-egfp)/Tg(flk1:mCherry) embryo showing the putative primary lumen (arrows) forming between the cellular junctions. The time format is minutes:seconds.

Expression Data

Genes:	cdh5 EGFP mCherry
Antibody:	Ab1-cdh5
Fish:	is3Tg; is4Tg is3Tg; is5Tg
Anatomical Terms:	blood vessel endothelial cell adherens junction blood vessel endothelial cell nucleus intersegmental vessel blood vessel endothelial cell
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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