FIGURE

Fig. 3

ID

ZDB-FIG-100903-23

Publication

Dutta et al., 2010 - Kctd15 inhibits neural crest formation by attenuating Wnt/β-catenin signaling output

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Fig. 3

Kctd15 inhibits the output of canonical Wnt signaling. (A) foxd3 expression in control embryos. (B) Loss of foxd3 expression in wnt8.1 morphants. (C) Injection of Wnt8.1 MO together with Kctd15a/15b MOs rescued foxd3 expression. (D) Quantification of A-C; number of embryos (n) is given above bars. (E-J) foxd3 expression in embryos treated with LiCl (E-G). Arrow in G indicates expanded expression in 26/30 embryos. (H-J) LiCl did not rescue foxd3 expression in kctd15a mRNA-injected embryos (I, 40/40; J, 50/50; numbers indicate embryos with appearance as shown in the figure). (K-M) Inhibition of foxd3 by kctd15a (K) was rescued by β-cat* mRNA (M, 41/50 showing foxd3 expression in NC domain). β-cat* mRNA alone induced ectopic expression of foxd3 (L, arrow, 25/31). (A-C,E-M) 1-somite stage; dorsal views, anterior towards top. Scale bar: 100 μm.

Expression Data

Gene:	foxd3
Fish:	WT WT + MO1-wnt8a WT + MO1-kctd15a + MO1-kctd15b + MO1-wnt8a
Knockdown Reagents:	MO1-kctd15a MO1-kctd15b MO1-wnt8a
Anatomical Terms:	neural crest cell notochord
Stage:	1-4 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-wnt8a
Knockdown Reagent:	MO1-wnt8a
Observed In:	neural crest cell neural crest cell differentiation
Stage:	1-4 somites

Phenotype Detail

Acknowledgments

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