FIGURE

Fig. 3

ID

ZDB-FIG-100903-12

Publication

Jeong et al., 2010 - Inhibition of Plk1 induces mitotic infidelity and embryonic growth defects in developing zebrafish embryos

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Fig. 3

Plk1 depletion leads to prometaphase arrest in zebrafish embryos, accompanied by congression failure and mitotic spindle abnormality. (A) A comparison of the percentage of cells in the indicated stages of mitosis, as judged by the degree of chromosome condensation, nuclear envelope breakdown (NEBD), and chromosome arrangement relative to spindle microtubules. (B) Embryos injected with 0.5 pmol of control or plk1 ATG MO were co-immunostained with anti-α-tubulin and anti-γ-tubulin antibodies at 24 hpf. Red, α-tubulin; green, γ-tubulin. Arrows mark uncongressed chromosomes. (C) Analyses of single cells from the embryos of Fig. 2H (and supplemental Fig. 2). Scale bar, 5 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-plk1
Knockdown Reagent:	MO2-plk1
Observed In:	cell centrosome cell mitotic spindle mitotic cell cycle, embryonic mitotic centrosome separation mitotic metaphase chromosome alignment mitotic prometaphase mitotic spindle assembly
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 345(1), Jeong, K., Jeong, J.Y., Lee, H.O., Choi, E., and Lee, H., Inhibition of Plk1 induces mitotic infidelity and embryonic growth defects in developing zebrafish embryos, 34-48, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.