FIGURE

Fig. S4

ID

ZDB-FIG-100816-29

Publication

Rhodes et al., 2010 - Positive regulation of c-Myc by cohesin is direct, and evolutionarily conserved

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Fig. S4

Validation of the zebrafish-specific Rad21 antibody. Protein lysates were prepared from 20 wild type (WT), sibling (sibs) and rad21^nz171 homozygous mutant (mts) embryos at 27 h.p.f. A, Immunoblot showing Rad21 protein. B, α-tubulin loading control. Although there is less total protein in the mutant sample, the Rad21 protein is considerably more reduced in rad21^nz171 mutants than can be expected due to loading discrepancy alone. As expected, siblings, comprising a mix of wild type and rad21^nz171 heterozygotes, show intermediate levels of Rad21 protein. The band pattern detected, with the major band above 130kDa, is similar to that detected with commercial anti-human Rad21 antibodies.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 344(2), Rhodes, J.M., Bentley, F.K., Print, C.G., Dorsett, D., Misulovin, Z., Dickinson, E.J., Crosier, K.E., Crosier, P.S., and Horsfield, J.A., Positive regulation of c-Myc by cohesin is direct, and evolutionarily conserved, 637-649, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.