The dlx regulatory elements recapitulate endogenous dlx expression in the forebrain of the zebrafish at 48 hours postfertilization (hpf). A-F: Whole-mount in situ with probes against dlx1a (A,B), dlx2a (C,D), and gfp (E,F). E,F: Tg(dlx1a/2aIG:GFP) is expressed in the telencephalon, prethalamus, and hypothalamus. There is also expression in the pharyngeal arches (arrowhead), which recapitulates a small portion of the endogenous dlx arch expression domain. G,H: URE2 activity closely resembles the expression of Tg(dlx1a/2aIG:GFP) in the telencephalon, prethalamus, and hypothalamus and mimics dlx expression in these tissues. tel, telencephalon; pTh, prethalamus; hyp, hypothalamus; di, diencephalon; md, mandible; ba, branchial arches; GFP, green fluorescent protein. Scale bar = 100 μm.
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