FIGURE

Fig. 2

ID

ZDB-FIG-100723-18

Publication

Iida et al., 2010 - Metalloprotease-dependent onset of blood circulation in zebrafish

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Fig. 2

Injection of Metalloprotease Inhibitors into the Flow Disturbs the Onset of Blood Circulation

(A) Protocol for monitoring effects of metalloprotease inhibitors (orthophenanthroline or GM6001) on blood circulation. These reagents were injected into the flow together with dye-labeled dextran (cyan) at 24 hpf. Blood circulation was observed at 30 hpf.

(B and C) Effects of metalloprotease inhibitors and those of solvents (mock) on the onset of blood circulation were observed under a stereomicroscope.

(B) Effects of metalloprotease inhibitors were evaluated by counting embryos showing complete blood stagnation (black bars) and those with partial stagnation (gray bars).

(C) Abnormal accumulation of gata1:mRFP⁺ cells is indicated by white arrowheads. Note that metalloprotease inhibitor-injected embryos exhibit efficient flow of dye-labeled dextran (cyan). The effects of these inhibitors are transient, and blood circulation starts within a few hours, suggesting recovery of proteolytic activity. Scale bars represent 250 μm.

Expression Data

Genes:	EGFP mRFP
Fish:	ko05Tg; y1Tg
Condition:	chemical treatment: pharmaceutical
Anatomical Terms:	dorsal aorta nucleate erythrocyte posterior cardinal vein
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ko05Tg; y1Tg
Condition:	chemical treatment: pharmaceutical
Observed In:	blood circulation embryonic hemopoiesis
Stage:	Prim-15

Phenotype Detail

Acknowledgments

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