FIGURE

Fig. 5

ID

ZDB-FIG-100622-15

Publication

Feng et al., 2010 - Conservation and divergence of methylation patterning in plants and animals

Other Figures

Fig. 5

All Figure Page

Back to All Figure Page

Fig. 5

Decrease of CG methylation in mutants lacking Uhrf1. (A) Reactivation of a transcriptionally silenced transgene in zebrafish uhrf1 homozygous mutants. WT larvae (3 days postfertilization) carrying the silenced allele of the brain-specific transgene Gt(Gal4-VP16;UAS:EGFP) c269 GFP (off) do not show any GFP labeled cells in the brain (Left) due to methylation of CG sites in the multicopy UAS (33). On the contrary, GFP labeled cells resulting from reactivation of the silenced c269 transgene are readily detected in the brains of uhrf1 homozygous mutant larvae (Right). (B–D) Comparison of CG methylation levels between wild-type and uhrf1 mutant lines from zebrafish (B), mouse (C), and Arabidopsis (D). For Arabidopsis, met1 mutant is used as a control for virtual total loss of CG methylation. Tissues used are mouse embryonic stem cells, Arabidopsis flowers, and zebrafish embryos.

Expression Data

Gene:	EGFP
Fish:	c269Tg uhrf1^{hi272Tg/hi272Tg}; c269Tg
Anatomical Term:	brain
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	uhrf1^{hi272Tg/hi272Tg}
Observed In:	DNA (cytosine-5-)-methyltransferase activity
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA