FIGURE

Fig. 3

ID

ZDB-FIG-100621-7

Publication

Akhtar et al., 2009 - Use of Phospholipase A(2) for Antigen Retrieval in Zebrafish Whole-Mount Immunohistochemistry

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Fig. 3

Concentrations of PLA₂ are different for nuclear and cytoplasmic serotonin antigen retrieval. High concentrations of PLA₂ are required for retrieval of nuclear serotonin within the intestine (A) and pharynx (C) but disrupt serotonin within the axons (A). Overall axon structure is not disrupted at this PLA₂ concentration as shown by acetylated tubulin IHC (B). Low concentrations of proteinase K partially permeabilize the tissue (D), while in combination with lower concentrations of PLA₂, the full pattern of nuclear (arrow) and axon (arrowhead) staining is present (E). In all panels, anterior is left and posterior is right. Scale bar is 20M at top left corner.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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