Fig. 6

smu mutation enhances defects of retinal neurogenesis in ncad and nok mutants. (A–D) BrdU incorporation in wild-type retina (A) and smu (B), ncad (C) and ncad; smu mutant retinas (D) at 48 hpf. (E–H) Labeling of wild-type (E), smu (F), ncad (G) and ncad; smu mutant retinas (H) with anti-pH3 antibody at 48 hpf. In the ncad; smu double mutant, the boundary between the neural retina and brain was often unclear. Thus, ath5:GFP expression (green) was visualized for demarcating the boundary between the neural retina and the brain (H). Adjacent retinal sections of the same ncad; smu double mutant embryo were used for the analyses of BrdU incorporation (D) and anti-pH3 antibody labeling (H). (I) Ratio of number of BrdU-positive cells to total number of retinal cells in wild-type retina and smu, ncad, and ncad; smu mutant retinas. There is no statistically significant difference in BrdU incorporation level between ncad and ncad; smu mutant retinas (p > 0.05; Student’s t-test). (J) Number of pH3-positive cells per sectioned retina of wild type and smu, ncad, and ncad; smu mutants. The number is higher in the ncad; smu double mutant retina than in the ncad single mutant retina (*p < 0.05: Student’s t-test). (K–N) BrdU incorporation in wild-type retina (K) and smu (L), nok (M), and nok; smu mutant retinas (N) at 48 hpf. (O–R) Labeling of wild-type retina (O) and smu (P), nok (Q), and nok; smu mutant retinas (R) with anti-pH3 antibody at 48 hpf. (S) Ratio of number of BrdU-positive cells to total number of retinal cells in wild-type retina and smu, nok, and nok; smu mutant retinas. There is no statistically significant difference in BrdU incorporation level between nok and nok; smu mutant retinas (p > 0.05; Student’s t-test). (T) Number of pH3-positive cells per sectioned retina of wild type and smu, nok, and nok; smu mutants. The number is higher in nok; smu double mutant retinas than in nok single mutant retinas (*p < 0.05: Student’s t-test).