FIGURE

Fig. 5

ID

ZDB-FIG-100506-62

Publication

Sheng et al., 2010 - Deletion of the WD40 domain of LRRK2 in Zebrafish causes Parkinsonism-like loss of neurons and locomotive defect

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

WISH Analysis of the loss of TH+ cell in WD40 morphant and its rescue by over-expressing wild-type zLRRK2 or hLRRK2 as well as hLRRK2 mutant alleles.

(A) WISH analysis at 3 dpf shows the decreased TH expression in WD40 deletion morphant as well as the successful rescue by over-expressing either zLRRK2 or hLRRK2. The analysis also shows the decreased TH expression in the fish with over-expression of wild-type zLRRK2 and hLRRK2. This neurodegenerative phenotype could not be rescued by the treatment of L-dopa. (B) Quantification of rescue effect of zLRRK2 or hLRRK2 on TH+ cell loss in WD40 morphant. ***P<0.001 (unpaired Student′s t-test). (C) Quantification of rescue effect of hG2019S or hG2385R on TH+ cell loss in WD40 morphant. **P<0.01, ***P<0.001 (unpaired Student′s t-test).

Expression Data

Gene:	th
Fish:	WT WT + MO1-lrrk2 + MO2-lrrk2
Knockdown Reagents:	MO1-lrrk2 MO2-lrrk2
Anatomical Term:	diencephalon dopaminergic neuron
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-lrrk2 + MO2-lrrk2
Knockdown Reagents:	MO1-lrrk2 MO2-lrrk2
Observed In:	diencephalon dopaminergic neuron
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.