FIGURE

Fig. 8

ID

ZDB-FIG-100506-6

Publication

Cheesman et al., 2004 - Zebrafish and fly Nkx6 proteins have similar CNS expression patterns and regulate motoneuron formation

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Fig. 8

Nkx6.1 is required for zebrafish secondary motoneuron formation. (A) Nkx6.1 protein in 24 hpf wild-type spinal cord. (B) nkx6.1 MO-injected embryo has no detectable protein in spinal cord at 24 hpf. (C) Wild-type and (D) MO-injected 12 hpf embryos have the same pattern of isl1-positive PMNs. RBs are out of focus on each side of PMNs. (E) At 48 hpf, ventral spinal cord is full of Neurolin-positive SMNs. (F) MO-injected embryo has few remaining SMNs (75% of injected animals, n=565). The brightly-stained cuboidal cells are floorplate. (G) GABA antibody labels KA and VeLD interneurons in ventral spinal cord at 24 hpf. (H) In MO-injected embryos there is a dramatic increase in GABA-positive VeLD interneurons (n=21). Rostral to left in all images. A,B,E-H lateral views. C,D dorsal views Scale bar: 50 μm for A-D; 33 μm for E-H.

Expression Data

Genes:	alcama isl1a
Antibodies:	Ab1-GABA Ab1-nkx6 zn-5
Fish:	AB AB + MO1-nkx6.1
Knockdown Reagent:	MO1-nkx6.1
Anatomical Terms:	Kolmer-Agduhr neuron primary motor neuron Rohon-Beard neuron spinal cord spinal cord secondary motor neuron VeLD
Stage Range:	5-9 somites to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-nkx6.1
Knockdown Reagent:	MO1-nkx6.1
Observed In:	apoptotic process neuron fate commitment primary motor neuron secondary motor neuron VeLD
Stage Range:	5-9 somites to Long-pec

Phenotype Detail

Acknowledgments

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