FIGURE

Fig. 5

ID

ZDB-FIG-100504-27

Publication

Carney et al., 2010 - Genetic analysis of fin development in zebrafish identifies furin and hemicentin1 as potential novel Fraser Syndrome disease genes

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Fig. 5

Partially redundant roles of zebrafish Frem2/3 paralogues.

In situ hybridisation of embryos using probes against frem2b (A–C), and frem3 (D) at 24 hpf (A, D), 32 hpf (B), and 48 hpf (C). Expression of frem3 in the fin fold can be seen at 24 hpf (D), whilst expression at this site only commences at 48 hpf for frem2b (C). frem2b is also expressed in the blood islands at 32hpf (arrowhead, G) and in the pronephric ducts from 24 hpf (arrowheads in A; dorsal view). (E–N) Lateral views of 32 hpf WT embryos (E,G,J,L), bla^ta90/ta90 embryos (F,H,K,M) or pif^tm95/tm95 embryos (I,N) which are either uninjected (E,F,I), or injected with morpholinos targeting frem2b (G,H), frem3 (J,K,N) or a mix of morpholinos against frem2b and frem3 (L,M). Blistered regions of the fin and blood islands are highlighted by bars and arrows respectively which are coloured green in bla mutants, red in frem2b morphants and blue in pif mutants.

Expression Data

Genes:	frem2b frem3
Fish:	WT
Anatomical Terms:	intermediate cell mass of mesoderm median fin fold pronephric duct somite
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	fras1^tm95b/tm95b frem2a^ta90/ta90 WT + MO1-frem2b WT + MO1-frem3 fras1^tm95b/tm95b + MO1-frem3 frem2a^ta90/ta90 + MO1-frem2b frem2a^ta90/ta90 + MO1-frem3 WT + MO1-frem2b + MO1-frem3 frem2a^ta90/ta90 + MO1-frem2b + MO1-frem3
Knockdown Reagents:	MO1-frem2b MO1-frem3
Observed In:	blood island medial fin morphogenesis median fin fold
Stage:	Prim-15

Phenotype Detail

Acknowledgments

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