FIGURE

Fig. 4

ID

ZDB-FIG-100429-11

Publication

Gates et al., 2010 - The transcriptional repressor REST/NRSF modulates hedgehog signaling

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Fig. 4

CyA mediated attenuation of Hh signaling is enhanced by Rest knockdown. Lateral views of 26 hpf control (A, C, E, G) and rest morphants (B, D, F, H) embryos stained for nkx2.2a (A–D) or pax3a (E–H). Embryos were incubated in control media (A, B, E, F) or 1.5 μM cyclopamine (CyA) media from 6 hpf on (C, D, G, H). rest morphants incubated in control media show a modest increase in nkx2.2a expression (B, compare to control, A) and a modest decrease in pax3a (F, compare to control, E) grown under the same conditions. 1.5 μM CyA decreases nkx2.2a expression (C) and modestly expands pax3a (G) in control embryos. Rest knockdown produces a greater reduction in nkx2.2a expression, and a greater expansion of pax3a, then CyA treatment alone.

Expression Data

Genes:	nkx2.2a pax3a
Fish:	WT WT + MO1-rest
Condition:	chemical treatment: Cyclopamine
Knockdown Reagent:	MO1-rest
Anatomical Terms:	hindbrain neural tube dorsal region hindbrain neural tube ventral region spinal cord neural tube ventral region
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 340(2), Gates, K.P., Mentzer, L., Karlstrom, R.O., and Sirotkin, H.I., The transcriptional repressor REST/NRSF modulates hedgehog signaling, 293-305, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.