FIGURE

Fig. 5

ID

ZDB-FIG-100427-40

Publication

Iwase et al., 2007 - The X-linked mental retardation gene SMCX/JARID1C defines a family of histone H3 lysine 4 demethylases

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Fig. 5

Recognition of H3K9me3 by a PHD Finger of SMCX

(A) Histone-peptide binding assay. Two PHD fingers of SMCX, referred as PHD1 and PHD2, were fused to GST and purified from bacteria. Biotinylated histone peptides shown in a panel were incubated with purified PHD fingers, and pulled down by streptavidin agarose. After washing, the bound GST fusion proteins were visualized by western blot analysis using anti-GST antibody. Among the differently methylated histone peptides that we tested, only H3K9me3 peptide bound PHD1. No detectable binding was found for PHD2.

(B) A388P mutation associated with X-linked mental retardation affects PHD1 binding to H3K9me3. GST-fused wild-type and mutant PHD1 carrying A388P were incubated with H3K9me3 peptide and washed four times, and the bound fractions were analyzed by western blot analysis.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Cell, 128(6), Iwase, S., Lan, F., Bayliss, P., de la Torre-Ubieta, L., Huarte, M., Qi, H.H., Whetstine, J.R., Bonni, A., Roberts, T.M., and Shi, Y., The X-linked mental retardation gene SMCX/JARID1C defines a family of histone H3 lysine 4 demethylases, 1077-1088, Copyright (2007) with permission from Elsevier. Full text @ Cell