Fig. 2

mbp+ cells are exclusively expressed in oligodendrocytes. A-F: All images are transverse sections of the spinal cord (A-D) and hindbrain (E,F) of 5 dpf Tg(mbp:egfp) zebrafish, dorsal up. A: Labeling with an anti-MBP antibody to mark oligodendrocyte myelin processes. Arrows indicate enhanced green fluorescent protein-positive (EGFP⁺) cell bodies and arrowheads indicate MBP⁺, EGFP⁺ myelin processes. B: Labeling with an anti-Sox10 antibody to mark OPCs and oligodendrocytes. Arrows indicate Sox10⁺, EGFP⁺ oligodendrocytes in the white matter and arrowheads indicate Sox10⁺, EGFP^- OPCs in the gray matter. C,D: Labeling with anti-Hu and anti-Blbp antibodies to mark neurons and radial glia, respectively. Arrows indicate EGFP⁺ oligodendrocytes. E,F: Labeling of hindbrain sections with anti-Sox10 and anti-Hu antibodies to detect oligodendrocytes and neurons, respectively. All EGFP⁺ cells are Sox10⁺ and Hu^- oligodendrocytes. Scale bar = 20 μm in A-D; 40 μm in E,F.