Fig. 3

aldh1a2^um22 and aldh1a2ⁱ²⁶ mutant embryos retain some endoderm gene expression at 24 and 30 hpf.

DMSO treated wild type embryos (A, E, I, M), DEAB-treated wild type embryos (B, F, J, N), embryos from an incross of aldh1a2^um22 heterozygotes (C, G, K, O) and embryos from an incross of aldh1a2ⁱ²⁶ heterozygotes (D, H, L, P) were assayed for expression of ins at 24 hpf (A–D; black arrows indicate residual expression), pdx1 at 24 hpf (E–H; black arrows indicate residual expression), hhex at 30 hpf (I–L; residual expression is indicated in pancreas (arrow) and liver (arrowhead)) and prox1 at 30 hpf (M–P; residual expression is indicated in liver (arrowhead)). Embryos are in dorsal view with anterior to the left. See Table 2 for quantification.