FIGURE

Fig. 2

ID

ZDB-FIG-091217-50

Publication

Hammond et al., 2009 - Two populations of endochondral osteoblasts with differential sensitivity to Hedgehog signalling

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Fig. 2

Mutants with altered Hh signalling show no differences in the number of osterix-positive cells in dermal bone elements. (A) Confocal stacks of the cleithra of osterix-nuclear GFP fish at 4 dpf. Inset image on left is a merged image of Tg(osx:nuGFP) (green), Alazarin Red and brightfield images. (B) Confocal stacks of the operculum of osterix-nuclear GFP fish at 4 dpf. Inset image on left is a merged image of Tg(osx:nuGFP) (green), Alazarin Red and brightfield images. (C) Quantification of the number of osterix-positive nuclei in the cleithrum (below) and operculum (above) at 3 and 4 dpf. Data is shown as mean±s.d. taken of at least four fish per genotype. *P<0.05 versus wild type. wt, wild type.

Expression Data

Gene:	GFP
Fish:	zf132Tg ptch2^{hu1602/hu1602}; zf132Tg
Anatomical Terms:	cleithrum osteoblast opercular flap osteoblast
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ihha^{hu2131/hu2131} ptch1^tj222/tj222 ptch2^{hu1602/hu1602} ptch2^{hu1602/hu1602}; zf132Tg
Observed In:	cleithrum osteoblast opercular flap osteoblast
Stage Range:	Protruding-mouth to Day 4

Phenotype Detail

Acknowledgments

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