FIGURE

Fig. 3

ID

ZDB-FIG-091215-9

Publication

Tan et al., 2009 - Regulation of membrane progestin receptors in the zebrafish ovary by gonadotropin, activin, TGF-beta and BMP-15

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

Regulation of mPRα and mRPβ by hCG (H), activin-A (A) and TGF-β1 (T). (A) Mid- to late growth phase follicles were treated with control medium (C), hCG (100 ng/ml), activin-A (100 ng/ml), TGF-β1 (10 ng/ml), for 12 and 18 h, and proteins were extracted from intact follicles and subjected to Western blotting. Equal loading was confirmed by Western blotting probed with an anti-α-tubulin antibody. (B) Histograms of normalized densitometry data for the 18 h time point (mean ± S.E.M.) from four experiments. Different letters denote statistical significance (p < 0.05). (C) Follicles were treated with hCG, activin-A, and TGF-β1 for 18 h and follicular cell layer was removed. Proteins were extracted from oocytes and Western blotting performed using mPRβ and α-tubulin antibodies.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Molecular and Cellular Endocrinology, 312(1-2), Tan, Q., Zagrodny, A., Bernaudo, S., and Peng, C., Regulation of membrane progestin receptors in the zebrafish ovary by gonadotropin, activin, TGF-beta and BMP-15, 72-79, Copyright (2009) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.