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Fig. S9

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ZDB-FIG-091215-34

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Yue et al., 2009 - Beta-arrestin1 regulates zebrafish hematopoiesis through binding to YY1 and relieving polycomb group repression

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Fig. S9

YY1-Binding Mutant Screening and Characterization
(A) Co-immunoprecipitation analyses using human β-arrestin1 truncations. The C-terminal region of β-arrestin1 showed strong interactions with YY1, while β-arrestin1 1-360 displayed least binding with YY1. (B) 7M β-arrestin1 interacts with known β-arrestin1 binding partners as shown by co-immunoprecipitation assay in HEK293 cells. (C) 7M β-arrestin1 showed normal ability of ERK activation. HEK293 cells stably expressing β2AR were incubated with 20 μM H-89 (a well known PKA inhibitor which blocks G protein mediated ERK activation) and stimulated with or without 1 μM isoproterenol (Iso) for 15 minutes. Equal amount of cell lysates were separated by SDS-PAGE and analyzed for pERK by western blot.

Expression Data

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Antibody Labeling

Phenotype Data

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Acknowledgments

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Reprinted from Cell, 139(3), Yue, R., Kang, J., Zhao, C., Hu, W., Tang, Y., Liu, X., and Pei, G., Beta-arrestin1 regulates zebrafish hematopoiesis through binding to YY1 and relieving polycomb group repression, 535-546, Copyright (2009) with permission from Elsevier. Full text @ Cell