FIGURE

Fig. 7

ID

ZDB-FIG-091214-7

Publication

Bibliowicz et al., 2009 - Expanded progenitor populations, vitreo-retinal abnormalities, and Muller glial reactivity in the zebrafish leprechaun/patched2 retina

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Fig. 7

The number of proliferating CMZ progenitors is increased in lep/ptc2, while proliferation rate is unaffected. (A, B) BrdU ′pulse′ in sibling (A) and lep/ptc2 mutants (B). BrdU⁺cells are observed throughout the CMZ. (C, D) After an 8 hour ′chase′, BrdU⁺cells are observed at the CMZ, as well as within the retina of sibling (C) and lep/ptc2 mutants (D). E, F) PH3 labeling in sibling (E) and lep/ptc2 (F) at 64hpf. G) Quantification of BrdU⁺cells in sibling and lep/ptc2 after ′pulse-chase′ experiment. lep/ptc2 retinas contained 67% more BrdU⁺cells than sibling retinas at 64hpf. After an 8 hour ′chase′, the number of BrdU⁺cells increased by almost identical ratios in siblings (2.31-fold increase) and lep/ptc2 mutants (2.29-fold increase, ***p < 0.0001). H) Quantification of BrdU- and PH3-positive cells as a percentage of total retinal cells in lep/ptc2 and wild-type siblings at 64hpf. The proportion of both BrdU⁺and PH3⁺cells increased by similar ratios (1.34-fold increase, **p < 0.001 and 1.36-fold increase, *p < 0.01, respectively).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ptch1^tj222/tj222 (AB)
Observed In:	cell population proliferation ciliary marginal zone neuronal stem cell
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.