FIGURE

Fig. 3

ID

ZDB-FIG-091214-43

Publication

Vatine et al., 2009 - Light directs zebrafish period2 expression via conserved D and E boxes

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

The per2 minimal promoter is light-induced in vivo: quantification of pineal EGFP mRNA levels.

Tg(-0.43per2:EGFP)tlv1 embryos were entrained by exposure to two LD cycles. At the beginning of the third day of development, embryos were either exposed to light or kept in darkness. After 2 h (ZT 2 and CT 2) embryos were fixed and subjected to whole mount ISH for egfp mRNA. Signal intensities, determined using ImageJ software, revealed a 22-fold increase in pineal egfp mRNA levels after a 2 h light pulse (n = 16, p<0.01 by ANOVA) relative to pineal egfp expression in the DD controls. Error bars represent SE. Dorsal views of representative whole mounts are shown below.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Biol.