FIGURE

Fig. 1

ID

ZDB-FIG-091113-93

Publication

Hart et al., 2009 - Selective interaction between Trf3 and Taf3 required for early development and hematopoiesis

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Fig. 1

Taf3 is required for normal development and hematopoiesis in early zebrafish embryos. A: RT-PCR analysis monitoring expression of taf3 and, as a control, gapdh in zebrafish embryos at 0, 4, 8, and 24 hpf. B: Immunoblot analysis monitoring expression of Taf3 in zebrafish embryos at 0, 4, 6, 18, and 24 hpf. C: Whole-mount in situ hybridization with a riboprobe to taf3 at 6 and 24 hpf. D: Phenotypic analysis of embryos injected with a control, taf3, trf3, or cdx4 MO at 24 hpf. E: (Top) Whole-mount in situ hybridization with riboprobes to gata1 and pax2 (14 hpf). (Bottom) Fluorescence microscopy monitoring expression of a gata1:dsRed transgene at 30 hpf. F: Whole-mount in situ hybridization with riboprobes to scl and hbbe1 (18 hpf).

Expression Data

Genes:	DsRed gapdh gata1a hbbe1.1 pax2a taf3 tal1
Fish:	WT WT + MO1-taf3 sd2Tg sd2Tg + MO1-taf3
Knockdown Reagent:	MO1-taf3
Anatomical Terms:	common cardinal vein blood intermediate cell mass of mesoderm posterior lateral mesoderm whole organism
Stage Range:	1-cell to Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-taf3 WT + MO1-tbpl2 WT + MO3-cdx4 sd2Tg + MO1-taf3
Knockdown Reagents:	MO1-taf3 MO1-tbpl2 MO3-cdx4
Observed In:	blood island erythroid lineage cell embryo development hemopoiesis lateral plate mesoderm erythroid progenitor cell nucleate erythrocyte post-vent region trunk
Stage Range:	10-13 somites to Prim-15

Phenotype Detail

Acknowledgments

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