Fig. 6

Notch-responsivity marks centroacinar cells in adult zebrafish pancreas. (A) H & E stained sectioned from an adult zebrafish pancreas, demonstrating very similar histology to mammalian pancreas. (B and C) Confocal images of adult pancreata from Tg(ptf1a:eGFP)^jh1; Tg(Tp1bglob:hmgb1-mCherry)^jh11 fish. Acinar cells are easily identified by ptf1atransgene expression (green). The nuclei of PNCs are marked by red fluorescence. (C) High mag image of a single acinus. Apically localized zymogen granules (white arrowhead) appear as dark circles due to exclusion of GFP. The apical surface of the acinar cells surround three red nuclei of PNCs. Due to their location in the adult pancreas and their Notch responsivity, these cells are definitive centroacinar cells (scale bars = 10 μm). (D–F) Images from adult Tg(Tp1bglob:eGFP)^um14 pancreata. (D and E) Confocal images of immunofluorescent detection for GFP (green) and Carboxypeptidase A (CPA, red) performed on longitudinal cryo-sectioned pancreas (scale bars = 20 μm). (F) Immunogold detection of GFP on section through an acinus of Tg(Tp1bglob:eGFP)^um14 adult pancreas. EM clearly shows two centroacinar cells with irregular nuclei, surrounded by acinar cells (characterized by the presence of zymogen granules (►). Gold particles are localized to the centroacinar cells (see right inset enlargement to see gold particles) but not acinar cells (see left inset). Scale bar = 2 μm). (B–E) Nuclei are stained blue with Hoechst.