FIGURE

Fig. 1

ID

ZDB-FIG-091016-26

Publication

Anderson et al., 2009 - Loss of Dnmt1 catalytic activity reveals multiple roles for DNA methylation during pancreas development and regeneration

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Fig. 1

Initial formation and degeneration of the exocrine pancreas in dandelion (ddn) mutants. (A–C) Lateral views of WT (A), ddn^s872 (B), and ddn^s904 mutant (C) larvae at 100 hpf in the 2-color Liver, Insulin, acinar Pancreas (2CLIP) transgenic background (see Materials and methods). Acinar cells of the exocrine pancreas (xp) are green and hepatocytes (li) and pancreatic beta cells (b) are red. ddn mutants exhibit minimal acinar tissue, but overall larva morphology is only moderately affected. (D, E) Successive images of individual WT (D) and ddn^s872 mutant (E) larvae between 84 and 148 hpf in the 2CLIP; Tg(ptf1a:EGFP)^jh1 background. The exocrine pancreas and liver grow larger in WT, but degenerate in ddn mutants. Hepatocyte fragments are found in the circulation (arrowheads). (F–K) 3-D projections of confocal stacks showing composition of WT (F, H, J) and ddn^s872 mutant (G, I, K) endocrine pancreas at 100 hpf in Tg(ins:dsRed)^m1081 background. (F, G) A core of Tg(ins:dsRed)^m1081+ cells (red) is surrounded by a mantle of Glucagon+ (Gcg) cells (green). Extrapancreatic duct (epd) structure is intact. (H, I) Isl-1, and (J, K) Somatostatin (Sst) positive cells appear unaffected. Arrowheads in G, I, and M point to endocrine cells outside of the primary islet. (L, M) Nkx6.1 immunostaining reveals the presence of intra-pancreatic duct cells in both WT and dnmt1^s872 mutant larvae.

Expression Data

Genes:	DsRed EGFP GFP RFP
Antibodies:	Ab1-gcg Ab2-isl Ab2-nkx6 ab2-sst
Fish:	gz15Tg m1018Tg gz15Tg; jh1Tg m1018Tg; s854Tg dnmt1^s872/s872; gz15Tg dnmt1^s872/s872; gz15Tg; jh1Tg dnmt1^s872/s872; m1018Tg dnmt1^s872/s872; m1018Tg; s854Tg dnmt1^s904/s904; gz15Tg
Anatomical Terms:	blood hepatocyte endocrine pancreas endocrine pancreas pancreatic B cell exocrine pancreas exocrine pancreas acinar cell gall bladder liver hepatocyte pancreas pancreas glucagon secreting cell pancreas somatostatin secreting cell pancreatic duct
Stage Range:	Protruding-mouth to Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	dnmt1^s872/s872; gz15Tg dnmt1^s872/s872; gz15Tg; jh1Tg dnmt1^s872/s872; m1018Tg dnmt1^s872/s872; m1018Tg; s854Tg dnmt1^s904/s904; gz15Tg
Observed In:	endocrine pancreas pancreatic B cell endocrine pancreas somatostatin secreting cell exocrine pancreas liver pancreas pancreatic A cell pancreatic duct
Stage Range:	Protruding-mouth to Day 6

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 334(1), Anderson, R.M., Bosch, J.A., Goll, M.G., Hesselson, D., Dong, P.D., Shin, D., Chi, N.C., Shin, C.H., Schlegel, A., Halpern, M., and Stainier, D.Y., Loss of Dnmt1 catalytic activity reveals multiple roles for DNA methylation during pancreas development and regeneration, 213-223, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.