FIGURE

Fig. 3

ID

ZDB-FIG-090904-66

Publication

Arrington et al., 2009 - Extra-embryonic syndecan 2 regulates organ primordia migration and fibrillogenesis throughout the zebrafish embryo

Other Figures

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Fig. 3

Basement membrane and epithelial cell polarity are disrupted in yolk^sdc2MO embryos. (A,B) Transverse sections of wild-type (A) and yolk^sdc2MO (B) embryos at 22 somites reveal laminin deposition (white arrow) between the cardiac and ventral foregut primordia in wild-type embryos (3/3) and reduced laminin in yolk^sdc2MO embryos (5/6). Laminin, red; β-catenin, green. (C-F) Transverse sections of Tg(cmlc2:GFP) embryos at 22 somites immunostained for the cell-junction protein aPKC (green) and cardiac primordia (red). Magnified views of cardiac precursors (marked by rectangles in C and D) reveal apicolateral localization of aPKC protein (arrows) in noninjected embryos (E) and diminished aPKC in yolk^sdc2MO embryos (F).

Expression Data

Gene:	EGFP
Antibodies:	Ab1-prkcz AB9-GFP
Fish:	twu34Tg twu34Tg + MO4-sdc2
Knockdown Reagent:	MO4-sdc2
Anatomical Terms:	gut endothelial cell apicolateral plasma membrane heart primordium heart primordium apicolateral plasma membrane
Stage:	20-25 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO4-sdc2 AB + MO5-sdc2 twu34Tg + MO4-sdc2 twu34Tg + MO5-sdc2
Knockdown Reagents:	MO4-sdc2 MO5-sdc2
Observed In:	apical protein localization basement membrane organization
Stage:	20-25 somites

Phenotype Detail

Acknowledgments

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