FIGURE

Fig. 6

ID

ZDB-FIG-090814-21

Publication

Viktorin et al., 2009 - Emx3 is required for the differentiation of dorsal telencephalic neurons

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Fig. 6

A-H: Heat shock-induced Emx3 protein partially rescues emx3 morpholino antisense oligonucleotides (MO) -induced knockdown, whereas excessive Emx3 dorsalizes the telencephalon. A-C,E-G: Low levels of Emx3-myc-GFP expression (e3-GFP⁺), induced by heat shock at the 1- or 2-somite stages (10.5 hr, hs2s), 20-30 min after the onset of endogenous emx3 mRNA expression at bud stage, rescues the emx3MO-induced down regulation of tcf4l (A-C) and eomesa (eom., E-G). Later heat shock, between the 5- and 7-somite stages (11.5 hr, hs5s), does not rescue the phenotype (B,D). Higher levels of GFP expression after heat shock (e3-GFP⁺⁺), indicating higher levels of Emx3 activity, lead to dorsalized telencephalon as assessed by ventrally expanded expression of tcf4l (A,H). A-D,H: Frontal views, dorsal to the top, 25 hr; (E-G) Dorsal view, rostral to the left, 12-somite stage (15 hr). Scale bar = 100 μm.

Expression Data

Genes:	eomesa tcf4
Fish:	AB + MO2-emx3 + MO3-emx3 + MO4-tp53 b1202Tg/+ + MO2-emx3 + MO3-emx3 + MO4-tp53 (AB) b1202Tg/+ + MO4-tp53 (AB)
Condition:	heat shock
Knockdown Reagents:	MO2-emx3 MO3-emx3 MO4-tp53
Anatomical Term:	dorsal telencephalon
Stage Range:	10-13 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO2-emx3 + MO3-emx3 + MO4-tp53 b1202Tg/+ + MO2-emx3 + MO3-emx3 + MO4-tp53 (AB) b1202Tg/+ + MO4-tp53 (AB)
Condition:	heat shock
Knockdown Reagents:	MO2-emx3 MO3-emx3 MO4-tp53
Observed In:	dorsal telencephalon telencephalon
Stage Range:	10-13 somites to Prim-5

Phenotype Detail

Acknowledgments

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