oxytocin-like is downregulated in nsfst53 zebrafish following an acute osmolar challenge. (A) Whole-mount in situ hybridization in 5 dpf wild-type (WT) and nsfst53 control or morpholino-injected zebrafish (top panels) or following 24 h 10x salt treatment (middle panel) is shown. The decrease in expression of oxytocin-like (oxtl) in nsfst53 zebrafish exposed to 10x salt is noted (black arrow). TUNEL-positive cells around the gills in nsfst53 zebrafish following 10x salt treatment are shown (black arrowhead). Images are representative of at least four animals per genotype per treatment. The salt challenge was repeated twice. (B) Relative transcript levels are shown for oxtl in wild-type and nsfst53 zebrafish heads at 5 dpf, with or without 10x salt exposure (n = 8 each). Fold induction is relative to one control sample and taken to be 1.0. All other samples, including additional control and experimental samples, are normalized to this one wild-type sample. Variation in samples is shown by error bars (standard error of the mean). Statistical analyses were done by unpaired t-test. *P < 0.05.
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