Mutations in a zebrafish moz. (A) Positional cloning of the gene disrupted by the b719 mutation. The LG5 genomic region is schematized at the top, with informative genetic markers shown. 4T, 14T and 114T are polymorphisms in the T7 end of PACs 4O19, 14P16 and 114E16, respectively. Position of four PACs are shown below the genomic region, with the moz region expanded underneath. moz spans the non-recombinant interval, with exons on both non-recombinant PAC ends. Lesions are schematized in purple and shown in the chromatograms on the left side of this panel: b719 deletes one bp (cytosine) in exon 14 of moz. A C-to-T missense mutation in b999 introduces an early stop codon in the 16th exon. Positions of morpholino oligos (MOs) are shown in green (see Tables 1 and 2). (B) Schematic of protein domains of human, zebrafish wild type, b719 mutant and b999 mutant. Amino acid domains (Kitabayashi et al., 2001a): H, H15 nuclear localization; PHD, PHD fingers; basic; MYST HAT, MYST family histone acetyltransferase; acidic; serine rich; glutamate rich; methionine rich. The percent identity between human and wild-type zebrafish is listed for each region beneath human MOZ. The C-terminal transactivation domain of human MOZ (Champagne et al., 2001; Kitabayashi et al., 2001a) is labeled. The gray domain in b719 mutant is frame-shifted prior to translation stop. (C-E) Embryonic expression of moz in wild types at 28 hpf (C,D) and 48 hpf (E). Lateral views of head (C), head/trunk interface (D) and whole larva (E). At both stages, moz expression appears ubiquitous in cranial tissues, and has a diffuse posterior border of expression near the boundary (arrowhead in D,E) of the hindbrain and spinal cord. Expression at these stages is not detected in the trunk and tail. Scale bars: 50 μm.
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