FIGURE

Fig. S4

ID

ZDB-FIG-090616-2

Publication

Friedman et al., 2009 - MicroRNAs are essential for development and function of inner ear hair cells in vertebrates

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Fig. S4

Morpholino knockdown of miR-15a-1 using different MOs. (Left) Thirty-hpf embryos probed with miR-15a locked nucleic acid (LNA) probe to detect mature miRNAs. (Scale bar, 200 μm.) (Middle) Nomarski images of embryos at 30 hpf show presence of 2 otoliths in the inner ear; MMO-15a-1 morphants are indistinguishable from uninjected embryos (data not shown). (Right) Nomarski images of embryos at 55 hpf showing that miR-15a-1 morphants fail to show proper initiation of semicircular canal formation (image of MMO-15a-1 morphant ear at this stage is shown in Fig. 4B and Fig. S6B). Many embryos treated with MO-15a-1-S look normal at this stage; the embryo shown is representative of fewer than 10% that show the canal phenotype.

Expression Data

Gene:	mir15a-1
Fish:	WT
Anatomical Term:	whole organism
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-mir15a-1 WT + MO3-mir15a-1
Knockdown Reagents:	MO2-mir15a-1 MO3-mir15a-1
Observed In:	otolith semicircular canal semicircular canal morphogenesis
Stage Range:	Prim-15 to Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA