FIGURE

Fig. 5

ID

ZDB-FIG-090414-5

Publication

Marques et al., 2009 - Differential requirement for BMP signaling in atrial and ventricular lineages establishes cardiac chamber proportionality

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

Levels of BMP signaling affect cardiomyocyte number and proportion in a dose-dependent manner. (A–C) Frontal views of hearts at 48 h.p.f.; immunofluorescence as in Fig. 1. Scale bar represents 50 μm. Hearts depict examples from (A) an alk8CA mRNA-injected embryo displaying a v2–v3 class of severely ventralized body morphology (Kishimoto et al., 1997), (B) a wild-type embryo, and (C) an alk8(K232R) mRNA-injected embryo displaying a c4 class of severely dorsalized body morphology (Mullins et al., 1996). (D) Quantification of cardiomyocytes at 48 h.p.f., following injection of alk8CA or alk8(K232R) mRNA. Injected embryos were separated into previously described phenotypic classes based on body morphology. Injection of alk8CA mRNA causes a range of ventralization classes (v2–v3, v1, and Nv, meaning normal morphology) (Kishimoto et al., 1997). Injection of alk8(K232R) mRNA causes a range of dorsalization classes (Nc, meaning normal morphology, c2–c3 and c4) (Mullins et al., 1996). Graph indicates fold difference in mean values for each phenotypic class relative to wild-type embryos.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 328(2), Marques, S.R., and Yelon, D., Differential requirement for BMP signaling in atrial and ventricular lineages establishes cardiac chamber proportionality, 472-482, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.