Fig. 5

Comparison of mgo and noi^tu29a mutant phenotypes. A-D: Lateral view of 48-hpf embryos. noi^tu29a mutant with severe edema (arrowhead) (B). Wiltype (A), mgo (C), and mgo/+;noi/+ (D) did not show edema phenotype. E-H: wt1 expression in Wild-type, noi^tu29a, mgo, and mgo/+;noi/+ embryos at 30 hpf as indicated. Arrowheads mark the pronephric primordial. wt1 expression in the pronephric primordial was significantly reduced and caudally expanded in noi^tu29a (F), whereas expression in the mgo was normal (G). In the mgo/+;noi/+ embryos, wt1 expression was expanded slightly (H). I-L: Dorsal views of 24 hpf, showing proximal convoluted tubules (arrowheads) as marked by pax2a expression. In noi^tu29a, the proximal convoluted tubules were absent (J), whereas pax2a expression in the mgo and the mgo/+;noi/+ embryos were normal (K,L). M-P: Lateral view images of 24-hpf embryos showing smaller otic vesicles in noi^tu29a (N). Q-T: Dorsal views of 24-hpf embryos stained for pax2a expression in the otic vesicle. Brackets delineate ear size. The otic vesicle of noi^tu29a embryos (R) were smaller than the wild-type (Q), mgo (S), and mgo/+;noi/+ (T) embryos. U-X: Dorsal views of 48-hpf embryos stained with anti-ZN5 antibody. The optic chiasms are indicated by arrowheads. In the wild-type embryo (U), the optic axons from the eyes cross completely in the optic chiasm. In the noi^tu29a mutant (V), the optic axons from one eye ran into the axons from the other eye, whereas in mgo (W) and mgo/+;noi/+ (X) embryos, the optic axons were normal.