FIGURE

Fig. 5

ID

ZDB-FIG-090331-11

Publication

Nelson et al., 2009 - Retinal homeobox 1 is required for retinal neurogenesis and photoreceptor differentiation in embryonic zebrafish

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Fig. 5

Rx1 morphants but not rx2 morphants exhibit significant changes in retinal progenitor proliferation. (A–H) Retinal cryosections stained with anti-PH3, a marker for cells in M-phase, from embryos fixed at 25 hpf (A, B), 34 hpf (C, D), 53 hpf (E, F), and 72 hpf (G, H), injected with control MOs (A, C, E, G) or rx1-depleting MOs (B, D, F, H). Staining is markedly reduced in the rx1 morphants at the early times, but increased at the later times; arrow in D indicates disorganized mitotic cells that were occasionally observed in the morphants. I. Average numbers of PH3-positive cells as a function of treatment and time of assessment. Significant differences were evident following rx1 depletion for all times evaluated (***p < 0.001) but rx2 depletion caused no changes. Scale bar = 50 μm; error bars indicate ± 1 SD.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-rx1 + MO3-rx1
Knockdown Reagents:	MO2-rx1 MO3-rx1
Observed In:	cell population proliferation neural retina development
Stage Range:	Prim-5 to Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 328(1), Nelson, S.M., Park, L., and Stenkamp, D.L., Retinal homeobox 1 is required for retinal neurogenesis and photoreceptor differentiation in embryonic zebrafish, 24-39, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.