FIGURE

Fig. 1

ID

ZDB-FIG-090320-26

Publication

Stedman et al., 2009 - A functional interaction between Irx and Meis patterns the anterior hindbrain and activates krox20 expression in rhombomere 3

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Fig. 1

irx1b and irx7 are required for the expression of krox20 in r3. Embryos uninjected (ni; A, E, I, K, M) or injected with the morpholino against irx7 (Mo7; B,F) or against irx1b (Mo1b; C, G), coinjected with both morpholinos (Mo1bMo7) (D, H, J, L, N) or injected with Mo1b, Mosp7 and irx7 and gfp RNAs (O, P), were stained by ISH with probes indicated on the left and colour-coded. Panels A–D are lateral views of whole embryos, and panels E–P are dorsal views of flat-mounted embryos. Panel P is a high magnification of part of the hindbrain of a partially rescued morphant, which shows that krox20 expression in r3 is restored specifically in gfp and irx7 expressing cells (arrows). Rostral is toward the left. The embryonic stage is indicated at the bottom-right of each picture. Arrowheads in panels J, L, N indicate the absence of krox20 expression at the position of r3 in double Mo1bMo7 morphants.

Expression Data

Genes:	egr2b mafba otx2b
Fish:	WT WT + MO2-irx1b WT + MO2-irx7 WT + MO2-irx1b + MO2-irx7
Knockdown Reagents:	MO2-irx1b MO2-irx7
Anatomical Terms:	midbrain neural plate presumptive rhombomere 3 presumptive rhombomere 5 presumptive rhombomere 6
Stage Range:	1-4 somites to 5-9 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-irx1b + MO2-irx7
Knockdown Reagents:	MO2-irx1b MO2-irx7
Observed In:	hindbrain development
Stage Range:	1-4 somites to 5-9 somites

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 327(2), Stedman, A., Lecaudey, V., Havis, E., Anselme, I., Wassef, M., Gilardi-Hebenstreit, P., and Schneider-Maunoury, S., A functional interaction between Irx and Meis patterns the anterior hindbrain and activates krox20 expression in rhombomere 3, 566-577, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.