FIGURE

Fig. 3

ID

ZDB-FIG-090320-15

Publication

Kemp et al., 2009 - EphA4 and EfnB2a maintain rhombomere coherence by independently regulating intercalation of progenitor cells in the zebrafish neural keel

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Fig. 3

EphA4 and EfnB2a mediate hindbrain boundary sharpening during the neural keel stage. Whole mount (A) and 10 μm plastic sections (B–G) of RNA in situ hybridizations with a krox20/egr2b riboprobe. Anterior is to the left, dorsal or lateral views are indicated. Left panels show uninjected control embryos; right panels show stage-matched EphA4; EfnB2a double MO embryos. (A) Neural plate stage (N = 20); (B) neural plate to neural keel transition; (C,D) successive neural keel stages; (E,F) successive neural rod stages; (G) neural tube stage. Nc: r5-derived neural crest. Representative sections for each timepoint were chosen from at least 8 identically treated embryos. Scale bar: 50 μm.

Expression Data

Gene:	egr2b
Fish:	WT WT + MO1-epha4a + MO2-efnb2a
Knockdown Reagents:	MO1-epha4a MO2-efnb2a
Anatomical Terms:	neural crest presumptive rhombomere 3 presumptive rhombomere 5 rhombomere 3 rhombomere 5
Stage Range:	5-9 somites to 14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-epha4a + MO2-efnb2a
Knockdown Reagents:	MO1-epha4a MO2-efnb2a
Observed In:	rhombomere 3 rhombomere 5 rhombomere boundary formation
Stage Range:	5-9 somites to 14-19 somites

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 327(2), Kemp, H.A., Cooke, J.E., and Moens, C.B., EphA4 and EfnB2a maintain rhombomere coherence by independently regulating intercalation of progenitor cells in the zebrafish neural keel, 313-326, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.