Fig. 6

Reduced circulation in morphant embryos. (A, B) Lateral view of O-dianisidine stained 48 hpf (A) control and (B) morpholino injected embryos. Blood was present in the anterior blood vessels, the heart, the common cardinal vein, the dorsal aorta, and posterior cardinal vein. In the morphant embryo, blood was pooled as it exited the common cardinal vein and there was a reduction in blood cells in the anterior blood vessels. (C, D) Laser-scanning immunofluorescence microscopy images of transverse sections of 72 hpf embryo hearts. Fluorescence images with anti-E-cadherin antibody (red), a marker for epithelium and haematocytes, in (C) control morpholino injected embryo. Haematocyte numbers were reduced in (D) morphant heart. Hearts are outlined by white dotted lines. (E–H) The endothelial specific transgenic fli1:EGFP embryos injected with (G) control or (H) plakoglobin morpholino were analysed under light (E and F) and fluorescence microscopy (G and H) for vascular defects at 72 hpf. There was no alteration in vasculature in morphant embryos despite evident blood pooling (arrow).