Fig. 3

Phenotypic analysis of Prdm5 functions in zebrafish development.

A–F: Depletion of prdm5. Cyclopia and other axial mesendodermal defects in prdm5 morpholino injected zebrafish embryos. 48 hpf embryos either wild type (A,E) or injected (B: ATGmo; C: SBmo- 4ng; D: SBmo-8ng, F: Mix mo) are shown. Arrows in C, D and F point to mesendodermal defects. G–I: Overexpression of hPRDM5 rescues the morpholino phenotype. G: embryos injected with SBmo morpholino; H: Injections of 100 pg hPRDM5 mRNA in zebrafish embryos results in a “dorsalized” phenotype. I: Co-injection of prdm5 SBmo and hPRDM5 mRNA results in normal embryos. 48 hpf embryos are shown. J–L: Depletion of prdm5 enhances the mbl phenotype. Effect of prdm5 morpholino injection on the mbl phenotype. Injection of Mix mo in embryos derived from incrosses of mbl carriers. J) wt embryo; K) mbl homozygous mutants; L) mbl heterozygotes injected with Mix mo. All embryos analysed at 48 hpf.