FIGURE

Fig. 2

ID

ZDB-FIG-090113-10

Publication

Esterberg et al., 2009 - dlx3b/4b are required for the formation of the preplacodal region and otic placode through local modulation of BMP activity

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Fig. 2

Depletion of Chd reveals anti-BMP function of Dlx3b/4b. (A–D) Intermediate cell mass of the tail is increased in Dlx3b/4b morphants (B), reminiscent of a ventralization phenotype. Injection of a low dose of chd-mo results in a mild V1 ventralization phenotype (C). Knockdown of Chd and Dlx3b/4b increases the severity of ventralization (D). (E–M) Heightened BMP activity in Chd/Dlx3b/4b triple morphant embryos severely reduces otic expression of eya1 (E–G), pax2a (H–J), and tbx2b (K–M), while leaving expression in surrounding tissue intact. (A–D) are lateral views and (E–M) are dorsal views, with anterior to the left. All embryos are 24 hpf.

Expression Data

Genes:	eya1 pax2a tbx2b
Fish:	WT + MO1-chrd WT + MO5-dlx3b + MO5-dlx4b WT + MO1-chrd + MO5-dlx3b + MO5-dlx4b
Knockdown Reagents:	MO1-chrd MO5-dlx3b MO5-dlx4b
Anatomical Term:	otic vesicle
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-chrd WT + MO5-dlx3b + MO5-dlx4b WT + MO1-chrd + MO5-dlx3b + MO5-dlx4b
Knockdown Reagents:	MO1-chrd MO5-dlx3b MO5-dlx4b
Observed In:	intermediate cell mass of mesoderm post-vent region
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 325(1), Esterberg, R., and Fritz, A., dlx3b/4b are required for the formation of the preplacodal region and otic placode through local modulation of BMP activity, 189-199, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.