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Formation of the fourth ventricle choroid plexus in Gateways zebrafish transgenics.
A, at 24hpf GFP expression was absent at the roof of fourth ventricle; B, at 36hpf GFP-positive cells are present at the dorsal midline of the roof of fourth ventricle; C, the number of GFP-positive cells increased, they are separated from the roof plate posteriorly; D, ChP has formed at the dorsal midline at the ear level; E, F, ChP tightens. G, H, lateral and dorsal view of gfp expression pattern in transgenics. Compare that to i showing expression of zgc:66340 in dorsal view. A–F, dorsal view. Abbreviations: dChP – diencephalic ChP; ChP – choroid plexus of hindbrain, hb – hindbrain, mhb – midbrain-hindbrain boundary, r5 – rhombomere 5, rp – roof plate, tc – tela choroidea. In all figures scale bars = 50 μm unless otherwise indicated.
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