FIGURE

Fig. 7

ID

ZDB-FIG-080904-3

Publication

Owens et al., 2008 - Identification of genetic and chemical modulators of zebrafish mechanosensory hair cell death

Other Figures

All Figure Page

Back to All Figure Page

Fig. 7

Epistasis analysis of sentinel and protective compounds.
Neomycin dose-response relationship showing effects of 10 μM PROTO-1 against 100 μM or 200 μM neomycin exposure in wildtype and sentinel larvae. For each group, hair cells were pre-labeled with FM1-43FX. Animals were pretreated with PROTO-1 for 1 hour (or mock-treated), treated with neomycin and PROTO-1 for 1 hour, euthanized, and fixed. Hair cells of four neuromasts (left and right) were counted and the average number of hair cells per neuromast was determined. Number of hair cells in control animals (no PROTO-1, no neomycin) are shown with black bars, animals treated with only 100 μM or 200 μM neomycin are shown by solid colored bars, and animals treated with PROTO-1 and neomycin are shown by hatched colored bars. Error bars show 1 S.E.M. PROTO-1 and sentinel mutants show similar protection, and there is a small, statistically significant effect of the combined treatment of the mutation plus PROTO-1.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB cc2d2a^w38/w38 (AB)
Conditions:	chemical treatment: neomycin chemical treatment: neomycin
Observed In:	neuromast hair cell
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.