FIGURE

Fig. S2

ID

ZDB-FIG-080829-54

Publication

Kishi et al., 2008 - The identification of zebrafish mutants showing alterations in senescence-associated biomarkers

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Fig. S2

Morpholino-induced knockdown of nrs and terf2 in zebrafish embryos generates phenocopies of the corresponding mutants. (A) nrs morphants show yolk-opaque phenotypes starting at 2.5 dpf (with PTU), which is an exact phenocopy of the nrs mutant embryos which also manifested an obvious yolk-opaque substance. Upon SA-β-gal staining at 3 dpf, an extremely high level of induction was detected in the nrs morphants, in an identical manner to the SA-β-gal levels observed in the nrs mutants, while the control-injected embryos did not show any significant SA-β-gal activity as well as opaque yolks. (B) terf2 morphants (3.5 dpf) show robust SA-β-gal induction having relatively moderate but quite a similar morphological phenotype to the terf2 allele mutant, terfa^{hi1182/hi1182} which has a slightly weaker phenotype than terfa^{hi3678/hi3678}.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	terfa^{hi1182Tg/hi1182Tg} WT + MO1-spns1 WT + MO1-terfa
Knockdown Reagents:	MO1-spns1 MO1-terfa
Observed In:	cellular senescence eye head yolk
Stage Range:	Pec-fin to Protruding-mouth

Phenotype Detail

Acknowledgments

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