Fig. 6

Autonomous and non-autonomous roles for erbb3b in pigment pattern metamorphosis. (A) Wild-type → picasso chimeras frequently developed wild-type melanophores in stripes at high density anteriorly (arrows, left) but at lower density in the mid-trunk (small arrows, right; 75% of chimeras developed donor melanophores; chimeras with donor cells and total reared: n=24, 64, respectively). A wild-type midbody lateral line is misrouted as well. (B) Melanophores at high density anteriorly that are either donor-derived (EGFP⁺) or host-derived (EGFP^-). (C) Melanophores in the mid-trunk are more spread, which is typical at low density. In reciprocal picasso → wild-type chimeras, we did not observe donor metamorphic melanophores (n=7, 50). (D) Wild-type → nacre chimeras developed patches of donor-derived metamorphic melanophores that populated stripes (arrow) and scales (84% of chimeras developed metamorphic melanophores; n=75, 155). Persisting embryonic/early larval melanophores (arrowheads) are identifiable by location, large size and browner color (Quigley et al., 2004). (E) picasso → nacre chimeras developed melanophores (arrowheads), but did not develop metamorphic melanophores [79% of chimeras developed embryonic/early larval melanophores or fin melanophores (not shown); n=58, 195]. Donor cells in all chimera combinations contributed at similar frequencies to other derivatives, including muscle, epidermis, eye and neurons of the lateral line. Scale bars: in A, 500 μm; in B, 200 μm for B,C; in D, 1 mm for D,E.