FIGURE

Fig. 3

ID

ZDB-FIG-080715-4

Publication

Mei et al., 2008 - C1q-like inhibits p53-mediated apoptosis and controls normal hematopoiesis during zebrafish embryogenesis

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Fig. 3

Effect of the C1q-like knockdown on embryo development. (A) Embryo phenotypes observed under the microscope (a, c, e) and the head phenotypes stained with Alcian Blue (b, d, f). The arrows show the defected craniofacial phenotypes in the C1q-like morphant. Abbreviations: cb(I–V), first to fifth ceratobranchial; ch, ceratohyal; mk, Meckel's cartilage; pf, pectoral fin; pq, palatoquandrate. Each picture represents typical results out of three separate experiments (30 embryos in each experiment). (B, C) The statistical data of three independent experiments on C1q-like knockdown and C1q-like over-expression (B), as well as C1q-like mRNA rescue and p53 MO co-injection (B). Results are represented as mean ± SD of three separate experiments (60 embryos in each experiment). *p < 0.05. (D) Western blot detection of C1q-like knockdown during embryogenesis. The protein extracts from 30 embryos (28 hpf and 32 hpf) were analyzed by Western blot using the polyclonal anti-C1q-like antibody. A band of about 24 KD was not detected in C1q-like morphants. The picture represents typical result from three separate experiments.

Expression Data

Gene:	c1ql4l
Antibody:	ab1-c1ql4l
Fish:	WT WT + MO1-c1ql4l
Knockdown Reagent:	MO1-c1ql4l
Anatomical Term:	whole organism
Stage Range:	Prim-5 to Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-c1ql4l WT + MO4-tp53
Knockdown Reagents:	MO1-c1ql4l MO4-tp53
Observed In:	brain cell ceratobranchial cartilage ceratohyal cartilage embryonic viscerocranium morphogenesis midbrain hindbrain boundary whole organism
Stage Range:	Prim-5 to Day 4

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 319(2), Mei, J., Zhang, Q.Y., Li, Z., Lin, S., and Gui, J.F., C1q-like inhibits p53-mediated apoptosis and controls normal hematopoiesis during zebrafish embryogenesis, 273-284, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.