FIGURE

Fig. 4

ID

ZDB-FIG-080715-29

Publication

Liu et al., 2008 - In vivo time-lapse imaging delineates the zebrafish pituitary proopiomelanocortin lineage boundary regulated by FGF3 signal

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Fig. 4

Lineage specific suppression of PI POMC cells in FGF3 morphants. 1–2 cell stage POMC-GFP/PRL-RFP double transgenic embryos were either injected with 0.4 ng of FGF3 MO (B) or vehicle (A) and then cultured in routine medium. At 56 hpf, pituitary POMC-GFP/PRL-RFP expression patterns were analyzed in live embryos using confocal microscopy. In FGF3 morphant, POMC-GFP expressing cells are detected within the rostral pars distalis but not in the pars intermedia, whereas PRL-RFP expression is intact. Ventral pituitary view under high magnification (63x), anterior to the left. WT, wild type; MO, FGF3 morphant. RPD, rostral pars distalis; PI, pars intermedia. Scale bar, 50 μm.

Expression Data

Genes:	GFP RFP
Fish:	zf113Tg; zf44Tg zf113Tg; zf44Tg + MO1-fgf3
Knockdown Reagent:	MO1-fgf3
Anatomical Terms:	pars anterior pars intermedia
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	zf113Tg; zf44Tg + MO1-fgf3
Knockdown Reagent:	MO1-fgf3
Observed In:	adenohypophysis morphogenesis pars intermedia
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 319(2), Liu, N.A., Ren, M., Song, J., Ríos, Y., Wawrowsky, K., Ben-Shlomo, A., Lin, S., and Melmed, S., In vivo time-lapse imaging delineates the zebrafish pituitary proopiomelanocortin lineage boundary regulated by FGF3 signal, 192-200, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.