FIGURE

Fig. 5

ID

ZDB-FIG-080702-12

Publication

Mendelsohn et al., 2008 - The zebrafish embryo as a dynamic model of anoxia tolerance

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Fig. 5

The AMP-activated protein kinase (AMPK) pathway is activated by the inhibition of oxidative phosphorylation in zebrafish embryos. A: Immunoblot analysis of AMPK phosphorylation before and after 2 min in KCN, with increasing KCN doses, or with increasing time in anoxia. B: Phosphorylation of Acetyl-CoA Carboxylase (ACC) with time in KCN or anoxia. C: Phosphorylation of p70-S6 Kinase (S6K) in KCN or anoxia. D: Seventy-two hour postfertilization (hpf) embryos were incubated in KCN and the timing of AMPK phosphorylation was assessed. E: Change in the AMP/ATP ratio after a 5 min inhibition of oxidative phosphorylation as described in the Experimental Procedures section. Error bars are ±SD. All experiments used 24 hpf embryos.

Expression Data

Antibodies:	Ab1-acac Ab1-prkaa Ab2-acac Ab2-prkaa Ab6-rps6k Ab8-rps6k
Fish:	AB
Conditions:	chemical treatment: potassium cyanide hypoxia
Anatomical Term:	whole organism
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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